HybriDetect for immunoassay development | immunoassay techniques
Do you need to develop a new immunoassay technique or method?
Milenia HybriDetect is a ready-to-use, universal test strip (dipstick), which is based on lateral flow technology using gold particles. The dipstick is designed to develop qualitative or quantitative rapid test systems for several analytes such as proteins, antibodies, or gene amplifications. The user needs to develop an analyte-specific solution, which contains a first detector (e.g. antibody, antigen, specific probe) labeled with FITC and a second one (e.g. antibody, primer) labeled with biotin. The sample containing the analyte is mixed with the developed analyte-specific solution, and then the dipstick is placed into the solution.
The complexed analyte, labeled with FITC and biotin, binds first to the gold-labeled FITC-specific antibodies in the sample application area of the dipstick. Driven by capillary action, the gold complexes diffuse through the membrane. Only the analyte captured gold particles will bound when they flow over the immobilized biotin-ligand molecules at the test band and generate there a red-blue band over the time. Uncaptured gold particles flow over the control band and will be fixed there by species-specific antibodies. With increasing incubation time, the formation of an intensely colored control band appears.
Package size: 100 tests FOR RESEARCH USE ONLY
Assay time: minutes (dependent on application)
Evaluation: By eye or with Milenia POCScan Reader
Sample volume: 100μL of sample
Please contact us if you would like to see the instructions for use for the Milenia Hybridetect kit.