PMN Elastase ELISA kit
96 well ELISA microplate
Incubation time: 2 h 20 min
Range: 15.6 - 1000 ng/ml
Sensitivity: 0.2 ng/ml
Sample size: 10 µl
Sample type: plasma, seminal fluid, exudate, BALF, CSF
Substrate: TMB 450nm
The human immune system reacts with an inflammatory response to attacks from invading pathogens (fungi, bacteria and viruses) or damaged tissue following trauma or surgery. PMN granulocytes play an important role as primary defence cells in this inflammatory reaction. Bloodstream mediators such as cytokines, leukotrienes, complement factors, bacterial endotoxins, clotting and fibrinolysis factors attract and stimulate these PMN cells to phagocytise and destroy the invading pathogens.
PMN granulocytes use proteinases to digest these agents and tissue debris. One of these proteinases is PMN elastase which is localised in the azurophilic granules of the PMN granulocytes.
During phagocytosis of the foreign substances these enzymes are also partially excreted into the extracellular matrix, where the activity of PMN elastase is regulated by inhibitors (especially the α1 -proteinase inhibitor, α1 -PI. An overwhelming release of PMN elastase, however, can exceed the inhibitory potential of the α1-proteinase inhibitor. Thus, enzymatically active PMN elastase, together with simultaneously produced oxidants radicals can cause local tissue injury.
Due to the bloodstream and lymphatic system, however, α1-PI is delivered subsequently and eventually is able to form a complex with all excreted elastase. Therefore, the concentration of the PMN elastase/α1 -PI complex correlates with the released PMN elastase and can be used as a measure for the activity of granulocytes during an inflammatory response.
Primarily, determinations of PMN elastase find its application in observation of the course of trauma, shock and sepsis. Further indications are in the areas of hemodialysis, obstetrics, joint diseases, effusions of sport injuries, intestinal infection, pancreatitis and cystic fibrosis.