Human Leptin (highly sensitive) - Research Use Only
The recessive obesity mutation, ob, identified in 1950, results in profound obesity and type II diabetes as part of a syndrome that resembles morbid obesity in humans. Freidman’s group at Rockefeller University originally identified the obesity gene that encodes for Leptin in 1994. Leptin is a 16 kDa secreted protein produced by the ob gene.
Adipose tissue produces Leptin and releases it into the bloodstream. As fat deposits grow, blood Leptin levels tend to increase. It has been suggested that Leptin acts as a lipostat, increasing as fat gets deposited into adipocytes. It is also found that the protein acts as a hormone instructing the brain to stop food consumption and to increase activity. The protein has also been shown to signal and probably control the onset of puberty. In addition, recent study shows Leptin acts as a potent inhibitor of bone formation through central nervous system. This ELISA kit has a high sensitivity for measuring Human Leptin.
The ELISA kit measures both native and recombinant human leptin.
96 well ELISA microplate
Incubation time: 2 h 40 min
Range: 15.6 - 1000 pg/mL
Sensitivity: 2.13 pg/mL
Diluted Sample size: 100 µl
Sample type: EDTA plasma serum and cell culture fluid
Substrate: TMB 450nm